Tracking glycosylation in live cells using FTIR spectroscopy

FTIR spectroscopic imaging of live cancer cells

FTIR spectroscopic imaging allows the visualization of the distribution of chemical components in cells. However, obtaining images of living cells is difficult because of the strong absorption of water in the mid-infrared region. The use of attenuated total reflection (ATR) FTIR spectroscopic imaging provides a possibility to study tissues or live cells in an aqueous environment.[1] This approa...

Tracking bio-molecules in live cells using quantum dots.

Single particle tracking (SPT) techniques were developed to explore bio-molecules dynamics in live cells at single molecule sensitivity and nanometer spatial resolution. Recent developments in quantum dots (Qdots) surface coating and bio-conjugation schemes have made them most suitable probes for live cell applications. Here we review recent advancements in using quantum dots as SPT probes for ...

ftir spectroscopy in biological research

Single mRNA tracking in live cells.

Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of in...

High Resolution Ftir Spectroscopy

In FT-ICLAS, the aim is to improve the absorption detection sensitivity. The experiment combines IntraCavity Laser Absorption Spectroscopy (ICLAS) with FTIR spectroscopy. ICLAS is a well known instrumental technique in which a sample is inserted in a cell placed inside a broadband, multimode laser cavity. The laser is operating in pulsed mode and intracavity absorption rises as the laser wave b...